u6 promoter expresses sgrna Search Results


u6 promoter sgrna encoding plasmid  (Addgene inc)
95
Addgene inc u6 promoter sgrna encoding plasmid
U6 Promoter Sgrna Encoding Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+promoter+expresses+sgrna/us12319938-238-24-34?v=Addgene+inc
Average 95 stars, based on 1 article reviews
u6 promoter sgrna encoding plasmid - by Bioz Stars, 2026-07
95/100 stars
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arabidopsis u6-26 promoters with sgrna  (GenScript corporation)
90
GenScript corporation arabidopsis u6-26 promoters with sgrna
Construction of binary vectors for genome editing in soybean. Cas9 fused with a single nuclear localization signal (NLS) is expressed with a Cauliflower mosaic virus 35s (CaMV 35s) promoter. Synthetic guide RNA <t>(sgRNA)</t> is derived using U6 promoters. ( a <t>)</t> <t>Arabidopsis</t> thaliana U6-26 promoter ( b ) Glycine max U6-10 promoter. Sequences containing two Bsa I sites are located between the U6 promoter and the sgRNA scaffold. These sequences can be easily replaced with a gene-specific sgRNA seed. LB: left border; RB: right border.
Arabidopsis U6 26 Promoters With Sgrna, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+promoter+expresses+sgrna/pmc04448504-129-7-10?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
arabidopsis u6-26 promoters with sgrna - by Bioz Stars, 2026-07
90/100 stars
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sgrna vectors containing oryza sativa u6 promoter  (GenScript corporation)
90
GenScript corporation sgrna vectors containing oryza sativa u6 promoter
Strategy for targeted mutagenesis of sugarcane MgCh and confirmation of In-vitro cleavage activity of sgRNAs. (A) Schematic representation of sugarcane MgCh locus and sgRNAs' target sites, sgRNA1 targeting nts 731–750 and sgRNA2 targeting nts 1223–1242. Mutations at <t>sgRNA</t> target site 1 would disrupt the Nco I restriction recognition site. Exons are indicated with green boxes, and introns with yellow boxes. (B) In vitro cleavage assay to validate sgRNA activity, the (810 nts) MgCh PCR amplicon is digested by ribonucleoprotein complex (RNP) of Cas9 and either sgRNA1 or sgRNA2 into ~581 and 229 nts or 701 and 109 nts, respectively. (C) Map of sugarcane gene editing plasmid (pMGE); Two sgRNAs are monoscistronically expressed <t>under</t> <t>Oryza</t> sativa U6 promoter, npt II is under transcriptional control of cauliflower mosaic virus (CaMV) 35S promoter and CaMV terminator, Cas9 is under transcriptional control of CaMV 35S promoter and Sorghum bicolor HSP18 terminator. Protospacer adjacent motifs (PAMs) are indicated in red font.
Sgrna Vectors Containing Oryza Sativa U6 Promoter, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+promoter+expresses+sgrna/pmc08525377-75-0-16?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
sgrna vectors containing oryza sativa u6 promoter - by Bioz Stars, 2026-07
90/100 stars
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grna expression plasmids prgen-u6-sgrna  (ToolGen Incorporated)
90
ToolGen Incorporated grna expression plasmids prgen-u6-sgrna
Strategy for targeted mutagenesis of sugarcane MgCh and confirmation of In-vitro cleavage activity of sgRNAs. (A) Schematic representation of sugarcane MgCh locus and sgRNAs' target sites, sgRNA1 targeting nts 731–750 and sgRNA2 targeting nts 1223–1242. Mutations at <t>sgRNA</t> target site 1 would disrupt the Nco I restriction recognition site. Exons are indicated with green boxes, and introns with yellow boxes. (B) In vitro cleavage assay to validate sgRNA activity, the (810 nts) MgCh PCR amplicon is digested by ribonucleoprotein complex (RNP) of Cas9 and either sgRNA1 or sgRNA2 into ~581 and 229 nts or 701 and 109 nts, respectively. (C) Map of sugarcane gene editing plasmid (pMGE); Two sgRNAs are monoscistronically expressed <t>under</t> <t>Oryza</t> sativa U6 promoter, npt II is under transcriptional control of cauliflower mosaic virus (CaMV) 35S promoter and CaMV terminator, Cas9 is under transcriptional control of CaMV 35S promoter and Sorghum bicolor HSP18 terminator. Protospacer adjacent motifs (PAMs) are indicated in red font.
Grna Expression Plasmids Prgen U6 Sgrna, supplied by ToolGen Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+promoter+expresses+sgrna/10__1074_slash_mcp__m116__062729-59-1-8?v=ToolGen+Incorporated
Average 90 stars, based on 1 article reviews
grna expression plasmids prgen-u6-sgrna - by Bioz Stars, 2026-07
90/100 stars
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dnas encoding sgrnas (trans-activating rna-crisper rna chimera) under the control of the u6 promoter  (GenScript corporation)
90
GenScript corporation dnas encoding sgrnas (trans-activating rna-crisper rna chimera) under the control of the u6 promoter
Strategy for targeted mutagenesis of sugarcane MgCh and confirmation of In-vitro cleavage activity of sgRNAs. (A) Schematic representation of sugarcane MgCh locus and sgRNAs' target sites, sgRNA1 targeting nts 731–750 and sgRNA2 targeting nts 1223–1242. Mutations at <t>sgRNA</t> target site 1 would disrupt the Nco I restriction recognition site. Exons are indicated with green boxes, and introns with yellow boxes. (B) In vitro cleavage assay to validate sgRNA activity, the (810 nts) MgCh PCR amplicon is digested by ribonucleoprotein complex (RNP) of Cas9 and either sgRNA1 or sgRNA2 into ~581 and 229 nts or 701 and 109 nts, respectively. (C) Map of sugarcane gene editing plasmid (pMGE); Two sgRNAs are monoscistronically expressed <t>under</t> <t>Oryza</t> sativa U6 promoter, npt II is under transcriptional control of cauliflower mosaic virus (CaMV) 35S promoter and CaMV terminator, Cas9 is under transcriptional control of CaMV 35S promoter and Sorghum bicolor HSP18 terminator. Protospacer adjacent motifs (PAMs) are indicated in red font.
Dnas Encoding Sgrnas (Trans Activating Rna Crisper Rna Chimera) Under The Control Of The U6 Promoter, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+promoter+expresses+sgrna/pm28646206-209-12-17?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
dnas encoding sgrnas (trans-activating rna-crisper rna chimera) under the control of the u6 promoter - by Bioz Stars, 2026-07
90/100 stars
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dcas9-vp64 and u6-sgrna-cmv-mcherry expression cassettes  (Applied StemCell Inc)
90
Applied StemCell Inc dcas9-vp64 and u6-sgrna-cmv-mcherry expression cassettes
Strategy for targeted mutagenesis of sugarcane MgCh and confirmation of In-vitro cleavage activity of sgRNAs. (A) Schematic representation of sugarcane MgCh locus and sgRNAs' target sites, sgRNA1 targeting nts 731–750 and sgRNA2 targeting nts 1223–1242. Mutations at <t>sgRNA</t> target site 1 would disrupt the Nco I restriction recognition site. Exons are indicated with green boxes, and introns with yellow boxes. (B) In vitro cleavage assay to validate sgRNA activity, the (810 nts) MgCh PCR amplicon is digested by ribonucleoprotein complex (RNP) of Cas9 and either sgRNA1 or sgRNA2 into ~581 and 229 nts or 701 and 109 nts, respectively. (C) Map of sugarcane gene editing plasmid (pMGE); Two sgRNAs are monoscistronically expressed <t>under</t> <t>Oryza</t> sativa U6 promoter, npt II is under transcriptional control of cauliflower mosaic virus (CaMV) 35S promoter and CaMV terminator, Cas9 is under transcriptional control of CaMV 35S promoter and Sorghum bicolor HSP18 terminator. Protospacer adjacent motifs (PAMs) are indicated in red font.
Dcas9 Vp64 And U6 Sgrna Cmv Mcherry Expression Cassettes, supplied by Applied StemCell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+promoter+expresses+sgrna/us12285496-816-6-22?v=Applied+StemCell+Inc
Average 90 stars, based on 1 article reviews
dcas9-vp64 and u6-sgrna-cmv-mcherry expression cassettes - by Bioz Stars, 2026-07
90/100 stars
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sgrna sequences expressed under the control of u6 promoter  (VectorBuilder GmbH)
90
VectorBuilder GmbH sgrna sequences expressed under the control of u6 promoter
Strategy for targeted mutagenesis of sugarcane MgCh and confirmation of In-vitro cleavage activity of sgRNAs. (A) Schematic representation of sugarcane MgCh locus and sgRNAs' target sites, sgRNA1 targeting nts 731–750 and sgRNA2 targeting nts 1223–1242. Mutations at <t>sgRNA</t> target site 1 would disrupt the Nco I restriction recognition site. Exons are indicated with green boxes, and introns with yellow boxes. (B) In vitro cleavage assay to validate sgRNA activity, the (810 nts) MgCh PCR amplicon is digested by ribonucleoprotein complex (RNP) of Cas9 and either sgRNA1 or sgRNA2 into ~581 and 229 nts or 701 and 109 nts, respectively. (C) Map of sugarcane gene editing plasmid (pMGE); Two sgRNAs are monoscistronically expressed <t>under</t> <t>Oryza</t> sativa U6 promoter, npt II is under transcriptional control of cauliflower mosaic virus (CaMV) 35S promoter and CaMV terminator, Cas9 is under transcriptional control of CaMV 35S promoter and Sorghum bicolor HSP18 terminator. Protospacer adjacent motifs (PAMs) are indicated in red font.
Sgrna Sequences Expressed Under The Control Of U6 Promoter, supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+promoter+expresses+sgrna/bio_rxiv__2023__03__17__533041-251-20-12?v=VectorBuilder+GmbH
Average 90 stars, based on 1 article reviews
sgrna sequences expressed under the control of u6 promoter - by Bioz Stars, 2026-07
90/100 stars
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atg5-specific sgrna expression vectors prgen_human-atg5_u6_sg_3  (ToolGen Incorporated)
90
ToolGen Incorporated atg5-specific sgrna expression vectors prgen_human-atg5_u6_sg_3
Strategy for targeted mutagenesis of sugarcane MgCh and confirmation of In-vitro cleavage activity of sgRNAs. (A) Schematic representation of sugarcane MgCh locus and sgRNAs' target sites, sgRNA1 targeting nts 731–750 and sgRNA2 targeting nts 1223–1242. Mutations at <t>sgRNA</t> target site 1 would disrupt the Nco I restriction recognition site. Exons are indicated with green boxes, and introns with yellow boxes. (B) In vitro cleavage assay to validate sgRNA activity, the (810 nts) MgCh PCR amplicon is digested by ribonucleoprotein complex (RNP) of Cas9 and either sgRNA1 or sgRNA2 into ~581 and 229 nts or 701 and 109 nts, respectively. (C) Map of sugarcane gene editing plasmid (pMGE); Two sgRNAs are monoscistronically expressed <t>under</t> <t>Oryza</t> sativa U6 promoter, npt II is under transcriptional control of cauliflower mosaic virus (CaMV) 35S promoter and CaMV terminator, Cas9 is under transcriptional control of CaMV 35S promoter and Sorghum bicolor HSP18 terminator. Protospacer adjacent motifs (PAMs) are indicated in red font.
Atg5 Specific Sgrna Expression Vectors Prgen Human Atg5 U6 Sg 3, supplied by ToolGen Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+promoter+expresses+sgrna/pmc05082771-286-2-22?v=ToolGen+Incorporated
Average 90 stars, based on 1 article reviews
atg5-specific sgrna expression vectors prgen_human-atg5_u6_sg_3 - by Bioz Stars, 2026-07
90/100 stars
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pac103 pbneo u6 sgrna expression  (Addgene inc)
90
Addgene inc pac103 pbneo u6 sgrna expression
Strategy for targeted mutagenesis of sugarcane MgCh and confirmation of In-vitro cleavage activity of sgRNAs. (A) Schematic representation of sugarcane MgCh locus and sgRNAs' target sites, sgRNA1 targeting nts 731–750 and sgRNA2 targeting nts 1223–1242. Mutations at <t>sgRNA</t> target site 1 would disrupt the Nco I restriction recognition site. Exons are indicated with green boxes, and introns with yellow boxes. (B) In vitro cleavage assay to validate sgRNA activity, the (810 nts) MgCh PCR amplicon is digested by ribonucleoprotein complex (RNP) of Cas9 and either sgRNA1 or sgRNA2 into ~581 and 229 nts or 701 and 109 nts, respectively. (C) Map of sugarcane gene editing plasmid (pMGE); Two sgRNAs are monoscistronically expressed <t>under</t> <t>Oryza</t> sativa U6 promoter, npt II is under transcriptional control of cauliflower mosaic virus (CaMV) 35S promoter and CaMV terminator, Cas9 is under transcriptional control of CaMV 35S promoter and Sorghum bicolor HSP18 terminator. Protospacer adjacent motifs (PAMs) are indicated in red font.
Pac103 Pbneo U6 Sgrna Expression, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+promoter+expresses+sgrna/pm30185813-252-19-27?v=Addgene+inc
Average 90 stars, based on 1 article reviews
pac103 pbneo u6 sgrna expression - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


Construction of binary vectors for genome editing in soybean. Cas9 fused with a single nuclear localization signal (NLS) is expressed with a Cauliflower mosaic virus 35s (CaMV 35s) promoter. Synthetic guide RNA (sgRNA) is derived using U6 promoters. ( a ) Arabidopsis thaliana U6-26 promoter ( b ) Glycine max U6-10 promoter. Sequences containing two Bsa I sites are located between the U6 promoter and the sgRNA scaffold. These sequences can be easily replaced with a gene-specific sgRNA seed. LB: left border; RB: right border.

Journal: Scientific Reports

Article Title: Targeted mutagenesis in soybean using the CRISPR-Cas9 system

doi: 10.1038/srep10342

Figure Lengend Snippet: Construction of binary vectors for genome editing in soybean. Cas9 fused with a single nuclear localization signal (NLS) is expressed with a Cauliflower mosaic virus 35s (CaMV 35s) promoter. Synthetic guide RNA (sgRNA) is derived using U6 promoters. ( a ) Arabidopsis thaliana U6-26 promoter ( b ) Glycine max U6-10 promoter. Sequences containing two Bsa I sites are located between the U6 promoter and the sgRNA scaffold. These sequences can be easily replaced with a gene-specific sgRNA seed. LB: left border; RB: right border.

Article Snippet: Arabidopsis U6-26 and soybean U6-10 promoters with sgRNA were synthesized (Genscript, Nanjing, China) ( and ) and cloned into pUC57-Kan vectors to generate pUC57-AtU6-26-sgRNA and pUC57-GmU6-10-sgRNA plasmids, respectively.

Techniques: Virus, Derivative Assay

Strategy for targeted mutagenesis of sugarcane MgCh and confirmation of In-vitro cleavage activity of sgRNAs. (A) Schematic representation of sugarcane MgCh locus and sgRNAs' target sites, sgRNA1 targeting nts 731–750 and sgRNA2 targeting nts 1223–1242. Mutations at sgRNA target site 1 would disrupt the Nco I restriction recognition site. Exons are indicated with green boxes, and introns with yellow boxes. (B) In vitro cleavage assay to validate sgRNA activity, the (810 nts) MgCh PCR amplicon is digested by ribonucleoprotein complex (RNP) of Cas9 and either sgRNA1 or sgRNA2 into ~581 and 229 nts or 701 and 109 nts, respectively. (C) Map of sugarcane gene editing plasmid (pMGE); Two sgRNAs are monoscistronically expressed under Oryza sativa U6 promoter, npt II is under transcriptional control of cauliflower mosaic virus (CaMV) 35S promoter and CaMV terminator, Cas9 is under transcriptional control of CaMV 35S promoter and Sorghum bicolor HSP18 terminator. Protospacer adjacent motifs (PAMs) are indicated in red font.

Journal: Frontiers in Genome Editing

Article Title: Multiallelic, Targeted Mutagenesis of Magnesium Chelatase With CRISPR/Cas9 Provides a Rapidly Scorable Phenotype in Highly Polyploid Sugarcane

doi: 10.3389/fgeed.2021.654996

Figure Lengend Snippet: Strategy for targeted mutagenesis of sugarcane MgCh and confirmation of In-vitro cleavage activity of sgRNAs. (A) Schematic representation of sugarcane MgCh locus and sgRNAs' target sites, sgRNA1 targeting nts 731–750 and sgRNA2 targeting nts 1223–1242. Mutations at sgRNA target site 1 would disrupt the Nco I restriction recognition site. Exons are indicated with green boxes, and introns with yellow boxes. (B) In vitro cleavage assay to validate sgRNA activity, the (810 nts) MgCh PCR amplicon is digested by ribonucleoprotein complex (RNP) of Cas9 and either sgRNA1 or sgRNA2 into ~581 and 229 nts or 701 and 109 nts, respectively. (C) Map of sugarcane gene editing plasmid (pMGE); Two sgRNAs are monoscistronically expressed under Oryza sativa U6 promoter, npt II is under transcriptional control of cauliflower mosaic virus (CaMV) 35S promoter and CaMV terminator, Cas9 is under transcriptional control of CaMV 35S promoter and Sorghum bicolor HSP18 terminator. Protospacer adjacent motifs (PAMs) are indicated in red font.

Article Snippet: sgRNA vectors containing Oryza sativa U6 promoter were designed and custom synthesized in the pUC57 backbone (Genscript, NJ, USA) to generate pUCMg12.

Techniques: Mutagenesis, In Vitro, Activity Assay, Cleavage Assay, Amplification, Plasmid Preparation, Control, Virus